SubtiBank SubtiBank
yfjN [2019-07-25 09:45:28]
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yfjN [2019-07-25 09:45:28]

putative tRNA-dihydrouridine synthase 2
locus
BSU_08030
pI
5.38
mw
36.83 kDa
protein length
325 aa Sequence Blast
gene length
978 bp Sequence Blast
function
tRNA modification
product
putative tRNA-dihydrouridine synthase 2
essential
no
synonyms
dusC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

    • Gene

    Coordinates
    876,426 → 877,403

    The protein

    Catalyzed reaction/ biological activity

  • uridine in tRNA + NADPH + H+ → 5,6-dihydrouridine in tRNA + NADP+
  • 5,6-dihydrouridine in tRNA + NAD+ → uridine in tRNA + H+ + NADH (according to UniProt)
  • 5,6-dihydrouridine in tRNA + NADP+ → uridine in tRNA + H+ + NADPH (according to UniProt)

    • Protein family

  • Dus family (with [protein|CE45D56BC413555AF4E7914B5307AD967A358452|YacF], according to UniProt)

    • Paralogous protein(s)

  • [protein|CE45D56BC413555AF4E7914B5307AD967A358452|YacF]

    • [SW|Cofactors]

  • FMN or FAD [Pubmed|21635694]

    • Structure

  • [PDB|3B0P] (from Thermus thermophilus, 24% identity, from aa 12 ... 263) [pubmed|22123979]

    • Expression and Regulation

    Operons

    genes
    [gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]
    description
    [Pubmed|29794222]

    regulation

  • the leader mRNA is processed by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
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    Biological materials

    Mutant

  • MGNA-C277 (yfjN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2275 NBRP B. subtilis, Japan]
  • BKE08030 (Δ[gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCA
  • BKK08030 (Δ[gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCA

    • References

  • 25902496,22123979,29794222